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| Mitochondrial uncoupling does not influence the stability of the intracellularsignal activating plasma membrane calcium channels. J Duszyński, A Makowska, K Zabłocki Acta Biochim Polon 2002; 48(1):157-161 ICID: 146521 |
| IC™ Value: 6.80 |
| The effects of various concentrations of thapsigargin, a specific inhibitorof Ca2+-ATPase in the endoplasmic reticulum (ER) membrane, on calcium homeostasis in lymphoidal T cells(Jurkat) were investigated. Preincubation of these cells suspended in nominally calcium-free medium with0.1 microM thapsigargin resulted in a complete release of Ca2+ from intracellular calcium stores. Whenthe medium was supplemented with 3 mM CaCl2 the cells maintained constantly elevated level of cytosolicCa2+. However, thapsigargin applied at lower concentration produced only a partial depletion of the stores.For example, in the cells pretreated with 1 nM thapsigargin and suspended in calcium-free medium approximately75% of the calcium content was released from the intracellular stores. The addition of 3 mM CaCl2 tosuch cell suspension led to a transient increase in cytosolic calcium concentration, followed by a returnto a lower steady-state. This phenomenon, related to the refilling of the ER by Ca2+, allowed to estimatethe half-time for the process of cell recovery after activation of store-operated calcium channels. Bythis approach we have found that carbonyl cyanide m-chlorophenylhydrazone, which has been documentedto inhibit calcium entry into Jurkat cells, does not influence the stability of the intracellular signalinvolved in the activation of store-operated calcium channels. |
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ICID 146521 PMID 11440165 - click here to show this article in PubMed database |
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