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| Isolationand in vitro translation of leghaemoglobin mRNA from yellow lupinroot nodules. A B Legocki, A Konieczny Acta Biochim Polon 1978; 25(4):379-390 ICID: 147805 |
| IC™ Value: 5.00 |
| Messenger RNA for leghaemoglobin, extracted from polysomes of yellow lupinroot nodules, was purified by chromatography on oligo(dT1-cellulose followed by sucrose-gradient centrifugation.Leghaemoglobin mRNA activity was assayed in a cell-free protein synthesizing system derived from wheatgerm. The purified mRNA was sedimented in surcrose gradient in the 9S region which corresponds to a molecularweight of about 225,000. The poly(A) segment was estimated to be 66 nucleotides in length, based on hybridizationwith [3H]poly(U). Analysis of the translation product using immunoprecipitation and polyacrylamide-gelelectrophoresis in denaturing conditions revealed that the product made in vitro was identical with authenticleghaemoglobin. |
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ICID 147805 PMID 751363 - click here to show this article in PubMed database |
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