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IndexCopernicus Journal Abstract
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Nitric oxide modulates the physiological control of ciliary activity in themarine mussel Mytilus edulis via morphine: novel mu opiate receptor splice variants. Patrick Cadet Neuroendocrinol Lett 2004; 25(3):184-190 ICID: 400966 |
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| Article type: Original article | ||
| IC™ Value: 25.71 | ||
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| OBJECTIVES: The study sought to determine how dopamine controls ganglionicprocesses involved with modulating lateral cilia beating via the peripheral branchial nerve. METHODS:The lateral cilia found on the gill filaments exhibit metachronal ciliary beating determined stroboscopically.Novel opiate receptors were determined pharmacologically and demonstrated by RT-PCR and sequence analysisof total RNA from Mytilus edulis visceral ganglia. RESULTS: Dopamine applied to the visceral ganglioninhibits the activity of lateral cilia in a concentration and haloperidol sensitive manner. Morphineor DAMGO significantly enhances ciliary beating in a naloxone sensitive manner, whereas L-NAME, a nitricoxide synthase inhibitor, only antagonized morphine's action. SNAP, a nitric oxide donor, also enhancedlateral ciliary beating rates. Supporting the observation, i.e., morphine sensitive nitric oxide enhancementof ciliary beating and DAMGO insensitive, that two different mu opiate receptors are present in thistissue, a 602 bp fragment of the human micro 3 opiate receptor and a 935 bp fragment, designated micro4 have been demonstrated. CONCLUSIONS: The lateral epithelium of the gill is innervated by serotonergic,cilioexcitatory neurons and dopaminergic, cilioinhibitory neurons, originating in the visceral ganglion.This data supports previous reports that demonstrate inhibiting ganglionic dopamine release allows theserotonin signals to prevail uncontrolled, enhancing ciliary rates. Supporting the observation that twodifferent mu opiate receptors are present in this tissue, evidence is presented that identifies a 602bp fragment of the human micro 3 opiate receptor and a 935 bp fragment, designated micro 4. Overall,the data strongly suggests that the two alternatively spliced mu opiate receptors may be involved inthe physiological regulation of lateral ciliary activity in the visceral ganglia via dopamine and nitricoxide. | ||
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ICID 400966 PMID 15349083 - click here to show this article in PubMed database |
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